Education and training

2009 - Speciality degree in Immunology, Ministry of Health, Romania
1997-2004 - PhD degree in Immunobiology, Faculty of Biology, “Al. I. Cuza” University Iasi, Romania
2000-2002 - Master of Science in Immunology, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, UK
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, UK
1990-1995 - Bachelor degree in Biology, Faculty of Biology, “Al. I. Cuza” University Iasi, Romania

Work experience

1995-present - Biologist, Laboratory of Immunology and Genetics, St. Spiridon Hospital, Iasi, Romania
2002: July-October - Biologist, main operator of the Flow Cytometry Department, Max-Plank Institute for Immunobiology, Freiburg, Germany

Technical skills and competences

Expertise:
    -Flow Cytometry and cell sorting
    Training stages:
    -2002, training on FACS Canto, FACS Scan and MoFlo machines, Max Plank Institute for Immunobiology, Freiburg, Germany and
    -2004, training on FACS CantoII machine, Becton Dickinson Headquarters, Heidelberg, Germany
Flow-cytometry related applications:
    -immunophenotyping
    -apoptosis
    -cell proliferation
    -cell cycle
    -cytokine detection (beads/ intracellular)
    -cell signaling (phospho-protein detection)
Cell culture
ELISA, SDS-PAGE electrophoresis, Western blot

Most recent publications

1. Georgiana Grigore, Mihaela Zlei, Dragos Peptanariu, Iuliu Ivanov, Didona Ungureanu, Eugen Carasevici, In Vitro Hypoxic Preconditioning of Bone Marrow Stromal Cells Triggers ERK-Mediated Signaling and Growth of L363 Myeloma Cells, Romanian Review of Laboratory Medicine, Vol. 19, Nr. 1/4, Jan 2011

2. Mihaela Zlei, Georgiana Grigore, Dragos Peptanariu, Daniela Constantinescu, Corina Cianga, Eugen Carasevici, Monika Engelhardt, Alternative Fixation Method Improves Flow Cytometry- Assisted Phospho-Detection Competence Romanian Review of Laboratory Medicine, Vol. 18, Nr. 4/4, December 2010, p 69-79

3. Dăscălescu A, Zlei M, Grigore G, Dănăila C, Jitaru D, Carasevici E. [Prognostic significance of leukemia-associated phenotype in correlation with other biologic markers in acute myeloid leukemia patients]. Rev Med Chir Soc Med Nat Iasi. 2009 Oct-Dec;113(4):1176-81

4. Zlei M, Egert S, Wider D, Ihorst G, Wäsch R, Engelhardt M. Characterization of in vitro growth of multiple myeloma cells. Exp Hematol. 2007 Oct;35(10):1550-61


Under review at the Annals of Hematology Journal:

Mihaela Zlei, Georgiana Grigore, Dragos Peptanariu, Daniela Constantinescu, Corina Cianga, Ralph Wäsch, Eugen Carasevici, Monika Engelhardt; Constitutive Activation of Cell Growth and Survival-Related Intracellular Signaling Pathways in Multiple Myeloma Cells