Brief presentation of the research activity
The objectives of research lay within the fields of tumoral
immunology and oncology.. The true start took place in the early 80s and
culminated with in a doctorate entitled "Contributions to the study off the
relationship between the radiobiological effect and anti-tumoral immunity in
experimental cancer."(1983). The establishment of the Tumoral Immunology
Laboratory allowed access to grants after 1990 and approaching even more complex
topics: subgroup, specificity and anti-tumoral activity of NK cells,
proliferation mechanisms in malignant lymphomas, apoptosis as a regulating
factor of the cellular cycle in renal and mammary carcinomas , the profile of
cytokine secretion in diagnosing and monitoring the development of pulmonary
tuberculosis, characterizing lymphocytes subsets at the level of maternal-fetal
interface in normal and pathological pregnancy, new cellular and molecular in
patient centered therapy of malignant tumors - a genomic and proteomic approach;
selection criteria of therapeutic methods in secondary hepatic cancer:
clinical-biological and molecular fundamentals, biological consequences of
thermo-necrotizing by means of heating steam under pressure in hepatic
metastasis of colonic-rectal origin, the molecular study of the implication of
the BRCA genes in hereditary predisposition of the mammary and ovarian cancer in
certain risk families in the north-east of Romania, the study of the FLT 3
mutations in relapses from of acute myeloid leukemia,. epidemilogical research
on the influence of reproductive factors and on the polymorphisms of Cyp 1A1 and
p53 on the risk of genital neoplasms in the population in north-eastern Romania,
bone morrow microenvironment and focal adhesion in myelodysplasia in acute
leukemia, stromal cellular inflamatory fenotypes in breast cancer. Over the last
twenty years, equipement, technologies, the professionalism of the researchers
have been on an upward trajectory reaching the necessary level of
competitiveness in the globalised medical community.
Presentation of the developed research infrastructure
Until 1992 research infrastructure was rudimentary,
sometimes even makeshift in some places. In 1992, as the discipline of
Immunology was established, the Gr.T.Popa University of Medicine and Pharmacy
financed from its own funds the basic equipping of the laboratory at a value of
about 250 000 USD. This was followed by an equivalent investment sum in flow
cytometry equipments in the period immediately following. After that equipping
was exclusively based on grants awarded in internal competitions. All these
activities led to profiles of activity and research : histocompatibility
analyses for transplantations, cytogenetics and genetic diagnosis,
immunohistochemistry and FISH, cellular and molecular immunology , the
immunology of reproduction, cellular culture and flow cytometry. At present, as
a result of establishing on the basis of grants of the Interdisciplinary
Platform of Molecular Medicine, the following units have been equipped and put
into action: functional genomics, molecular imaging and tissue and flow
cytometry, cellular separations ant the study of stem cells.
Human resources have developed from student members of
"Journal Club" organized before 1989, to members of the immunology discipline in
1992, to which members of the discipline of genetics joined , thus making up The
Immunology and Genetics Laboratory of the St Spiridon University Hospital in
Iasi, (2002) with a strongly represented component of university research. The
Interdisciplinary Platform of Molecular Medicine which coexists in the same
place, has the following basic human resource: the executive director and head
of the laboratory, the coordinator of the department of molecular imaging, the
coordinator of the functional genomics unit, the coordinator of the
flow-cytometry unit, the coordinator of the cell separation and stem cell unit,
responsible for the genetic diagnostic activity, responsible for the molecular
epidemiology activity, responsible for molecular immunology activity,
responsible for coordinating formative activities.
International cooperation
- University “Auvergne”, Clermont-Ferrand, France
- Molecular Oncology Center “Jean Perrin”, Clermont-Ferrand, France
- Albert Ludwig University and Comprehensive Cancer Center University Hospital
Freiburg, Germany
- 774 INSERM Unit, Lille, France
International scientific events organized
- "John Humphrey" International Immunology Course Tumor Immunology, Aug.30 -
Sept.2, 1992, Iasi, Romania, Course organizers: Eugen Carasevici and Prof. Dr.
Klaus Eichmann, Romanian Society of Immunology and European Federation of
Immunological Societies
- John Humphrey"International Immunology Course "Self-Tolerance and
Self-Recognition", May 15-19 2000, Sinaia, Romania, Course organizers: Eugen
Carasevici and Prof. Dr. Klaus Eichmann, Romanian Society of Immunology and
European Federation of Immunological Societies
Published articles in ISI ranked journals
- Giusca SE, Eloae Zugun F, tarcoveanu E, Carasevici E, Amalinei C, Caruntu
ID: Immunohistochemical study of colorectal cancer liver metastases: the
immune/inflammatory infiltrate. Rom J Morphol Embryol. 2010;51(1):73-9.
- Ancuţa E, Ancuţa C, Cozma LG, Iordache C, Anghelache-Lupaşcu I, Anton E,
Carasevici E, Chirieac R. Tumor biomarkers in cervical cancer: focus on Ki-67
proliferation factor and E-cadherin expression. Rom J Morphol Embryol.
2009;50(3):413-8.
- Ancuţa E, Ancuţa C, Zugun-Eloae F, Iordache C, Chirieac R, Carasevici E.
Predictive value of cellular immune response in cervical cancer. Rom J Morphol
Embryol. 2009;50(4):651-5.
- Roxana-Georgiana Tauser, Florin Zugun-Eloae, Daniela Jitaru, Eugen
Carasevici Hipoxia-Inducible factor HIF-1alfa: Pharmacogenetic perspective for
Bevacizumab therapy Romanian Review of Laboratory Medicine 2009, vol.16, Nr. 3,:
7-12.
- Daniela Constantinescu, Carmen Cozmei Andreea Chiriac, Elena Mihalceanu,
Irina Dumitrascu, Eugen Carasevici. Investigarea citotoxicitatii periferice si
uterine in sarcinile normale si patologice de prim-trimestru. Revista Romana de
Medicina de Laborator,2008;11 (2):7-13.
- Cianga P, Cianga C, Plamadeala P, Branisteanu D, Carasevici E. The neonatal
Fc receptor (FcRn) expression in the human skin. Virchows Arch., 2007, oct, 451
(4):859-60.
- Diaconu C., Gavrilovici V., Ghenghe D., Dragomir C., Cozma L., Ciornea L.,
Carasevici E. Combined modality treatment for locally advanced breast cancer.
Acta Chirurgica Belgica, 2003, 103, 6, pp: 589-592.
- Petru Cianga, Corina Cianga, Laurette Cozma, E. Sally Ward, Eugen
Carasevici. The MHC class I related Fc receptor, FcRn, is expressed in the
epithelial cells of the human mammary gland. Human Immunology, 2003, vol. 64
(12), pp.: 1152-1159.
- Coman G., Moraru E., Butnaru F., Carasevici E., Apetrei C. Rhodococcus equi
systemic infection in an HIV-1 infected child, Clinical Microbiology and
Infection, 1998, vol. 4, no. 6, p 353-354.
- Carmen Cantemir, Carmen Cozmei, Brigitte Scutaru, Sergiu Nicoara, Eugen
Carasevici. p53 Protein expression in peripheral lymphocytes from atrazine
chronically intoxicated rats, Toxicology Letters, 1997, 93: p. 87-94.
- Lucian Negură, Eugen Carasevici, Anca Negură, Nancy Uhrhammer and Yves-Jean
Bignon, 2009. Identification of a recurrent BRCA1 mutation in a north-eastern
Romanian population. European Journal of Human Genetics, Volume 17, Supplement
2, p. 194. (Impact factor 2007, 4.003).
- Lucian Negură, Eugen Carasevici, Anca Negură, Nancy Uhrhammer and Yves-Jean
Bignon, 2009. Rapide PCR-RFLP screening for a novel BRCA2 mutation found in a
north-eastern Romanian population. FEBS Journal, Volume 276, Supplement 1, p.
331. (Impact factor 2007, 3.396).
- Lucian Negură, Eugen Carasevici, Anca Negură, Nancy Uhrhammer and Yves-Jean
Bignon, 2008. Preliminary genetic investigations of BRCA genes within HBOC
families in North-Eastern Romania. European Journal of Human Genetics, Volume
16, Supplement 2, p. 198. (Impact factor 2007, 4)
- Haulica, Human Physiology, Editura Medicala 2007, chapter 5 Internal Medium,
Immunity
- Liliana Padureanu, Carmen Cozmei, Eugen Carasevici: Immunopathology of
tuberculous infection in Tuberculoza. Mic tratat de pneumologie. Edit DAN, 2002
- Corneliu Diaconu, Lucian Miron, Eugen Carasevici, Breast Cancer, Editura
Cariatide, 1995
The expected impact of the results on the scientific community and on
society (at national and international level, novelty, etc.)
To establish, maintain, and run a modern molecular research
facility and create the founding premises of a synergistic environment for
research by pooling the varied expertises of the member scientists and
clinicians in the creation of a combined laboratory resource focused on
functional genomic technologies that are broadly applicable to studying the
basic biology of genes, proteins, cells and tissues, the complex interaction
between genes, normal and abnormal physiological functions and environment and
relevant for understanding normal biological processes and the way that these
are altered in specific diseases.
The expected impact on the scientific community include
advances in research for health, technology transfer, development of
biotechnology design projects, and the ability to maximise the benefits of
intellectual property.
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